Since trace textile fiber evidence is found at numerous crime scenes and plays an important role in linking a suspect to the respective scene, the current study examined the fluorescence of fiber dyes and fibers themselves, as well as a methodology for discriminating between fibers using room temperature fluorescence (RTF).
Initial systematic analysis was conducted on dye standards and extracts taken from fibers colored with the respective dyes of interest. Absorbance, excitation, and fluorescence spectra were compared between standards and extracts to determine the optimal area of the fiber to investigate dyes, fluorescent impurities, or the whole fiber. High performance liquid chromatography investigations were performed to obtain detailed information on the number of dye and fluorescent components present in extracts. Three-way Excitation Emission Matrix (EEM) data were found to give the greatest amount of spectral information and provide the highest level of discrimination. Successful discrimination between non-similar and similar fibers was achieved with the aid of second order MCR-ALS chemometric analysis. The level of discrimination obtained via RTF-EEM spectroscopy was sufficient to differentiate between two types of visually indistinguishable fibers and between fibers obtained from two separate cloths of the same material and colored with the same dye reagent. (Publisher Abstract Modified)
Downloads
Similar Publications
- Comparison of Privately and Publicly Operated Corrections Facilities in Kentucky and Massachusetts - Final Report
- Evaluation of Next Generation mtGenome Sequencing Using the Ion Torrent Personal Genome Machine PGM
- Prediction of Skull Fracture Risk for Children 0-9 Months Old Through Validated Parametric Finite Element Model and Cadaver Test Reconstruction